1L8F
Structure of 20K-endoglucanase from Melanocarpus albomyces at 1.8 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-24 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 47.440, 47.440, 156.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 1.800 |
| Rwork | 0.199 |
| R-free | 0.24700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.350 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.091 | 0.251 * |
| Total number of observations | 78044 * | |
| Number of reflections | 16780 * | |
| Completeness [%] | 95.6 | 88.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 298 | peg 8000, Na-acetate, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 12.5 (mM) | pH7.2 |
| 3 | 1 | drop | EDTA | 12.5 (mM) | |
| 4 | 1 | drop | sodium azide | 0.01 (%) | |
| 5 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.10 |
| 6 | 1 | reservoir | PEG8000 | 5 (%) |
