1L8D
Rad50 coiled-coil Zn hook
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-02-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.8321, 1.009 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.958, 77.868, 53.330 |
Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.200 |
Rwork | 0.225 |
R-free | 0.27800 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.790 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.076 * | 0.344 * |
Total number of observations | 133282 * | |
Number of reflections | 13119 | |
Completeness [%] | 88.0 | 62.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 300 | 100 mM Phosphate/Citrate, 40% Ethanol, 50 mM Zn-Acetate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
10 | 1 | reservoir | zinc acetate | 20 (mM) | |
2 | 1 | drop | phosphate | 20 (mM) | pH7.5 |
3 | 1 | drop | 200 (mM) | ||
4 | 1 | drop | EDTA | 0.1 (mM) | |
5 | 1 | drop | glycerol | 5 (%) | |
6 | 1 | drop | dithiothreitol | 2 (mM) | |
7 | 1 | reservoir | phosphate/citrate | 100 (mM) | pH4.2 |
8 | 1 | reservoir | ethanol | 40 (%) | |
9 | 1 | reservoir | glycerol | 10 (%) |