1L7Z
Crystal structure of Ca2+/Calmodulin complexed with myristoylated CAP-23/NAP-22 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-11-07 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.600, 40.600, 173.639 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.249 |
| Rwork | 0.239 |
| R-free | 0.27400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qiv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.070 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.083 * | |
| Total number of observations | 39942 * | |
| Number of reflections | 7815 * | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 96.9 | 99.2 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 4 * | PEG 1000, cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | CaM | 0.9 (mM) | |
| 2 | 1 | drop | peptide | 2.7 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | reservoir | PEG1000 | 36 (%) | |
| 5 | 1 | reservoir | cacodylate | 20 (mM) | pH5.5 |
