1L7T
Crystal Structure Analysis of the anti-testosterone Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1999-09-23 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.695, 88.419, 143.868 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 * - 2.100 |
Rwork | 0.213 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.340 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 * | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | 0.326 * |
Total number of observations | 120393 * | |
Number of reflections | 25386 * | |
Completeness [%] | 93.6 | 87.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 298 | Valjakka, J., (2002) J.Biol.Chem., 277, 4183. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | drop | Bis-Tris propane | 0.1 (M) | pH5.6 |
3 | 1 | drop | PEG3350 | 15 (%) |