1L7O

CRYSTAL STRUCTURE OF PHOSPHOSERINE PHOSPHATASE IN APO FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-12-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	36.888, 117.889, 117.361
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.200
Rwork	0.224
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f5s
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.200	2.200
Number of reflections	25996
<I/σ(I)>	18	5.5
Completeness [%]	98.2	85.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	20mM Zinc acetate, 10% PEG 3350, 0.1M cacodylate buffer, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K