1L7L
Crystal structure of Pseudomonas aeruginosa lectin 1 determined by single wavelength anomalous scattering phasing method
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-18 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 40.259, 72.351, 133.886 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.600 - 1.500 |
R-factor | 0.19262 |
Rwork | 0.192 |
R-free | 0.20020 |
Structure solution method | SAS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.301 |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.140 | |
Number of reflections | 31619 | |
<I/σ(I)> | 61 | 10 |
Completeness [%] | 99.3 | 96.3 |
Redundancy | 7.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |