1L7C
alpha-catenin fragment, residues 385-651
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9252, 0.9795, 0.9799, 1.0688 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.780, 105.290, 123.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.500 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.27300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 16.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.037 | 0.248 * |
| Number of reflections | 54918 | |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | sodium acetate, dithiothreitol, urea, sodium/potassium tartrate, isopropanol, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 80 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 700 (mM) | pH4.0 |
| 3 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 4 | 1 | reservoir | urea | 1.05 (M) | |
| 5 | 1 | reservoir | sodium potassium tartrate | 200 (mM) | |
| 6 | 1 | reservoir | isopropanol | 3 (%) |
