1L7A
structural Genomics, crystal structure of Cephalosporin C deacetylase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-06 |
Detector | SBC-2 |
Wavelength(s) | 0.9793, 0.9791, 0.9520 |
Spacegroup name | H 3 2 |
Unit cell lengths | 156.446, 156.446, 131.748 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.730 - 1.500 |
R-factor | 0.186 |
Rwork | 0.185 |
R-free | 0.18900 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 2.400 |
Data reduction software | HKL-2000 |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.078 | 0.325 |
Number of reflections | 96738 | |
<I/σ(I)> | 27.78 | 4.955 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 10.92 | 3.54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |