1L6Z

CRYSTAL STRUCTURE OF MURINE CEACAM1A[1,4]: A CORONAVIRUS RECEPTOR AND CELL ADHESION MOLECULE IN THE CEA FAMILY

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-06
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0715,1.0718,1.0534
Spacegroup name	P 31 2 1
Unit cell lengths	111.260, 111.260, 65.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 3.320
R-factor	0.297
Rwork	0.295
R-free	0.32900
Structure solution method	MAD and Molecular Replacement
Starting model (for MR)	PDB ENTRIES 1HNF AND 1E4J
RMSD bond length	0.011
RMSD bond angle	2.325
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.420
High resolution limit [Å]	3.320	3.320
Rmerge	0.073	0.371 *
Total number of observations	123640 *
Number of reflections	7127 *
<I/σ(I)>	17.3	3.7
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6 *	298	10% PEG 8000, 0.2 M magnesium acetate, 0.1 M cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.6 *	298	10% PEG 8000, 0.2 M magnesium acetate, 0.1 M cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	25 (mM)	pH7.6
2	1	drop		150 (mM)
3	1	drop	glycerol	5 (%)
4	1	drop	protein	0.5-1 (mg)
5	1	reservoir	PEG8000	10 (%)
6	1	reservoir	magnesium acetate	0.2 (M)
7	1	reservoir	cacodylate	0.1 (M)	pH6.4