1L6X

FC FRAGMENT OF RITUXIMAB BOUND TO A MINIMIZED VERSION OF THE B-DOMAIN FROM PROTEIN A CALLED Z34C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-05-15
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	C 2 2 21
Unit cell lengths	54.023, 124.774, 90.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.650
R-factor	0.206
Rwork	0.206
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1.95A Fc-Z34C complex collected at Chess. Fc was solved by molecular replacement using 2.8A 1FC1 Deisenhofer 1981
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (98.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	35573
<I/σ(I)>	17.8
Completeness [%]	95.4	88.7
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	20% PEG 550MME 0.1M NaOAc pH 5.5, 0.25M NaCl, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K