1L6R

Crystal Structure of Thermoplasma acidophilum 0175 (APC0014)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-08
Detector	SBC-2
Wavelength(s)	0.99187
Spacegroup name	I 2 2 2
Unit cell lengths	88.240, 99.244, 113.781
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.000 - 1.400
R-factor	0.173
Rwork	0.171
R-free	0.19500
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.058	0.425
Number of reflections	96123
<I/σ(I)>	13.2	3.9
Completeness [%]	97.6	100
Redundancy	7.7	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG3350, sodium HEPES, Calcium Chloride, Glycerol, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	22 (%)
2	1	drop		0.2 (M)
3	1	drop	sodium HEPES		pH7.5