1L5Z
CRYSTAL STRUCTURE OF THE E121K SUBSTITUTION OF THE RECEIVER DOMAIN OF SINORHIZOBIUM MELILOTI DCTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-09-30 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 57.129, 58.139, 168.824 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.700 - 2.000 |
Rwork | 0.196 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qkk |
RMSD bond length | 0.015 |
RMSD bond angle | 21.900 * |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.700 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.061 | 0.222 |
Total number of observations | 59135 * | |
Number of reflections | 19264 | |
<I/σ(I)> | 7.1 | 2.2 |
Completeness [%] | 98.9 * | 98.1 * |
Redundancy | 3.04 | 1.61 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 298 | HEPES, lithium sulfate, PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium succinate | 50 (mM) | pH5.6 |
2 | 1 | reservoir | 65-75 (mM) |