1L5H
FeMo-cofactor Deficient Nitrogenase MoFe Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.965 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 150.452, 191.922, 102.339 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.249 * |
Rwork | 0.249 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3min |
RMSD bond length | 0.008 |
RMSD bond angle | 1.380 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.088 | 0.440 |
Total number of observations | 1632866 * | |
Number of reflections | 60354 | |
<I/σ(I)> | 15.7 | 2.2 |
Completeness [%] | 91.6 | 87.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 9.5 | 298 | PEG 8000, CHES, pH 9.5, LIQUID DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 20 (%) | |
2 | 1 | reservoir | CHES | 0.1 (M) | pH9.5 |