1L4Z
X-RAY CRYSTAL STRUCTURE OF THE COMPLEX OF MICROPLASMINOGEN WITH ALPHA DOMAIN OF STREPTOKINASE IN THE PRESENCE CADMIUM IONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 118.400, 118.400, 134.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.800 |
| Rwork | 0.220 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ddj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 2.690 |
| High resolution limit [Å] | 2.800 * | 2.800 * |
| Rmerge | 0.064 | 0.380 * |
| Number of reflections | 14317 * | 1390 * |
| <I/σ(I)> | 43 | 5.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 20 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | Na acetate, cadmium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | SKalpha | 18 (mg/ml) | |
| 10 | 1 | reservoir | cadmium sulfate | 50 (mM) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | drop | urea | 0.4 (M) | |
| 4 | 1 | drop | uPgS741A | 16 (mg/ml) | |
| 5 | 1 | drop | HEPES | 20 (mM) | pH7.5 |
| 6 | 1 | drop | urea | 0.4 (M) | |
| 7 | 1 | drop | NaN3 | 0.02 (%(w/v)) | |
| 8 | 1 | reservoir | sodium acetate | 1.0 (M) | |
| 9 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
