1L4N
Crystal Structure of CobT complexed with 2-aminophenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 278 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.680, 89.900, 47.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.000 |
| Rwork | 0.167 |
| R-free | 0.19900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1d0s |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.480 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.060 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.055 | 0.136 |
| Number of reflections | 20587 | |
| <I/σ(I)> | 13.4 | 3.7 |
| Completeness [%] | 89.0 | 68.1 |
| Redundancy | 3.3 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | ammonium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP at 298K |
