1L3Z
Crystal Structure Analysis of an RNA Heptamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 25.740, 106.580, 30.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.010 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.18500 * |
RMSD bond length | 0.003 |
RMSD bond angle | 10.500 * |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.130 |
High resolution limit [Å] | 2.000 * | 2.010 |
Rmerge | 0.054 * | |
Number of reflections | 2840 * | |
Completeness [%] | 93.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | MPD, CaCl2, Cacodylate buffer, pH7.0 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | CaCl2 | ||
3 | 1 | 1 | Cacodylate buffer |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | oligonucleotide | 2 (mM) | single strand concentration |
2 | 1 | drop | sodium cacodylate | 40 (mM) | pH7.0 |
3 | 1 | drop | 24 (mM) | ||
4 | 1 | drop | MPD | 2 (%(v/v)) | |
5 | 1 | reservoir | MPD | 40 (%) |