1L3B
MT0146, THE PRECORRIN-6Y METHYLTRANSFERASE (CBIT) HOMOLOG FROM M. THERMOAUTOTROPHICUM, C2 SPACEGROUP W/ LONG CELL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97956 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.300, 59.200, 192.200 |
| Unit cell angles | 90.00, 108.60, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.650 |
| Rwork | 0.246 |
| R-free | 0.28590 * |
| Structure solution method | MOLECULAR REPLACEMEN |
| Starting model (for MR) | 1f38 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.670 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 2.690 |
| High resolution limit [Å] | 2.650 * | 2.640 |
| Rmerge | 0.109 * | 0.569 |
| Number of reflections | 89924 | |
| <I/σ(I)> | 1.78 | |
| Completeness [%] | 99.6 * | 99.9 |
| Redundancy | 2.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 25 * | PEG 8000, MGCL, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 10 (%(w/v)) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | Tris-Cl | 5 (mM) | pH7.5 |
| 5 | 1 | reservoir | PEG8000 | 16-22 (%) | |
| 6 | 1 | reservoir | 200 (mM) | ||
| 7 | 1 | reservoir | Tris-Cl | 10 (mM) | pH7.5 |
