1L3A

Structure of the plant transcriptional regulator PBF-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-29
Detector	BRANDEIS - B4
Wavelength(s)	1.100
Spacegroup name	P 21 21 21
Unit cell lengths	69.869, 89.807, 144.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.420 - 2.300
R-factor	0.216
Rwork	0.216
R-free	0.26700
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.228 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.420	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.081 *	0.370 *
Total number of observations	306528 *
Number of reflections	40505 *
Completeness [%]	98.4 *	89.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	eppendorf tubes *	8	277	Desveaux, D., (2002) Acta Crystallogr., Sect.D, 58, 296. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	12-15 (mg/ml)
2	1	1	Tris-HCl	20 (mM)	pH8.0