1L2W
Crystal Structure of the Yersinia Virulence Effector YopE Chaperone-binding Domain in Complex with its Secretion Chaperone, SycE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-03-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 72.845, 73.352, 74.263 |
Unit cell angles | 103.37, 109.18, 107.36 |
Refinement procedure
Resolution | 20.000 - 2.030 * |
R-factor | 0.249 * |
Rwork | 0.251 |
R-free | 0.27400 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jya |
RMSD bond length | 0.006 |
RMSD bond angle | 1.160 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.030 |
High resolution limit [Å] | 2.030 * | 2.030 * |
Rmerge | 0.058 | 0.490 * |
Number of reflections | 84804 | |
<I/σ(I)> | 18.22 | 1.77 |
Completeness [%] | 96.7 * | 96.7 * |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8 * | 18 * | PEG 8000, sodium tartrate, sodium acetate, dithiothreitol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 120 (mg/ml) | |
2 | 1 | 1 | Tris | 10 (mM) | pH8.0 |
3 | 1 | 1 | dithiothreitol | 1 (mM) | |
4 | 1 | 2 | PEG8000 | 18 (%(w/v)) | |
5 | 1 | 2 | sodium tartrate | 50 (mM) | |
6 | 1 | 2 | sodium acetate | 100 (mM) | pH4.6 |
7 | 1 | 2 | dithiothreitol | 1 (mM) |