1L2L
Crystal structure of ADP-dependent glucokinase from a Pyrococcus Horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 280 |
| Detector technology | CCD |
| Collection date | 2001-04-30 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.760, 74.730, 99.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.930 - 2.000 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.28000 * |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 21.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 * | 0.418 * |
| Number of reflections | 275056 * | |
| Completeness [%] | 99.1 * | 97.1 * |
| Redundancy | 8.3 * | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 20 * | PEG6000, LiSO4, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 9-13 (%) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | citrate | 0.1 (M) | pH3.6 |
