1L2G
Structure of a C-terminally truncated form of glycoprotein D from HSV-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-10 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 4 |
| Unit cell lengths | 131.416, 131.416, 83.265 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.850 |
| R-factor | 0.283 |
| Rwork | 0.278 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jma |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.674 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 29319 | |
| <I/σ(I)> | 11 | 2.7 |
| Completeness [%] | 87.1 | 67.3 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | Ammonium Sulfate, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
