1L2F

Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97923,0.97904,0.95372
Spacegroup name	P 43 21 2
Unit cell lengths	116.191, 116.191, 64.631
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.500
Rwork	0.223
R-free	0.29800
Structure solution method	MAD, SAD
RMSD bond length	0.011
RMSD bond angle	1.580 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.069 *	0.434 *
Number of reflections	29536 *	1408 *
Completeness [%]	99.8	96.8
Redundancy	10.5 *	8.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	ammonium sulfate, PEG400, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH7.5
3	1	drop	EDTA	1 (mM)
4	1	drop		0.2 (M)
5	1	drop	glycerol	5 (%)
6	1	reservoir	PEG400	2 (%)
7	1	reservoir	ammonium sulfate	2.0 (M)
8	1	reservoir	HEPES	0.1 (M)	pH7.5