1L2F
Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97923,0.97904,0.95372 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 116.191, 116.191, 64.631 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.500 |
| Rwork | 0.223 |
| R-free | 0.29800 |
| Structure solution method | MAD, SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.580 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.069 * | 0.434 * |
| Number of reflections | 29536 * | 1408 * |
| Completeness [%] | 99.8 | 96.8 |
| Redundancy | 10.5 * | 8.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | ammonium sulfate, PEG400, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | 0.2 (M) | ||
| 5 | 1 | drop | glycerol | 5 (%) | |
| 6 | 1 | reservoir | PEG400 | 2 (%) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 8 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
