1L2D
MutM (Fpg)-DNA Estranged Guanine Mismatch Recognition Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.906, 94.486, 104.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.217 * |
| Rwork | 0.217 |
| R-free | 0.25100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1l1t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.127 | 0.470 |
| Number of reflections | 30349 * | |
| <I/σ(I)> | 14 | 2.7 |
| Completeness [%] | 99.2 | 94 |
| Redundancy | 5.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 295 | PEG 8000, magnesium acetate, cacodylate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | magnesium acetate | ||
| 3 | 1 | 1 | cacodylate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | pH7.4 |
| 2 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | protein | 8 (mg/ml) | |
| 5 | 1 | reservoir | PEG8000 | 13-18 (%(w/v)) | |
| 6 | 1 | reservoir | sodium cacodylate | 100 (mg/ml) | pH7.5 |
| 7 | 1 | reservoir | magnesium acetate | 50 (mM) | |
| 8 | 1 | reservoir | beta-mercaptoethanol | 0.1 (%) |
