1L1N
POLIOVIRUS 3C PROTEINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-01-18 |
| Detector | SDMS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.260, 116.310, 47.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.213 * |
| Rwork | 0.213 |
| R-free | 0.25900 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.061 | 0.181 |
| Number of reflections | 26218 | 3446 * |
| <I/σ(I)> | 13.5 | 3 |
| Completeness [%] | 93.1 | 80.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | ammonium sulfate, glycerol, mercaptoethanol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17-20 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 20 (%(w/v)) | |
| 3 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 42 (%) | |
| 5 | 1 | reservoir | glycerol | 2 (%(v/v)) | |
| 6 | 1 | reservoir | beta-mercaptoethanol | (mM) | pH5.0 |
