1L1F

Structure of human glutamate dehydrogenase-apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2001-02-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	97.800, 98.800, 124.200
Unit cell angles	86.26, 70.28, 60.34

Refinement procedure

Resolution	8.000 - 2.700
R-factor	0.262 *
Rwork	0.262
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700	2.700 *
Rmerge	0.047 *	0.216 *
Number of reflections	94574 *	7168 *
Completeness [%]	90.5	54.8 *
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	PEG 8000, NaCl, sodium phosphate, sodium azide, octyl-b-glucopyranoside, methyl pentanediol, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	octyl-beta-glucopyranoside	1 (%(w/v))
2	1	reservoir	sodium azide	1 (mM)
3	1	reservoir		50 (mM)
4	1	reservoir	methyl pentanediol	8 (%(v/v))
5	1	reservoir	sodium phosphate	0.1 (M)	pH7.3
6	1	drop	enzyme	18 (mg/ml)
7	1	drop	sodium phosphate	0.1 (M)	pH7.0