1L0W

Aspartyl-tRNA synthetase-1 from space-grown crystals

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-11-25
Detector	MARRESEARCH
Wavelength(s)	0.91
Spacegroup name	P 21 21 21
Unit cell lengths	62.020, 156.100, 177.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.980 - 2.010
R-factor	0.217 *
Rwork	0.217
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	23.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000 *	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.057	0.235
Total number of observations	320200 *
Number of reflections	100854
<I/σ(I)>	17.3	2.16
Completeness [%]	93.3 *	95.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	298	Ammonium sulfate, Tris-HCl, EDTA, DTT, PMSF, pH 7.5, Under microgravity, temperature 298K
1	Vapor diffusion *	7.5	298	Ammonium sulfate, Tris-HCl, EDTA, DTT, PMSF, pH 7.5, Under microgravity, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	ammonium sulfate	2.2 (M)
3	1	drop	Tris-HCl	25 (mM)	pH7.5
4	1	drop	EDTA	10 (mM)
5	1	drop	dithiothreitol	1 (mM)
6	1	drop	PMSF	0.01 (%(m/v))