1L0S

Choristoneura fumiferana (spruce budworm) antifreeze protein isoform 337

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-08-09
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 65
Unit cell lengths	128.400, 128.400, 68.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 2.300
R-factor	0.227
Rwork	0.223
R-free	0.26400
Structure solution method	SAS
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.790	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.067 *	0.336 *
Total number of observations	709616 *
Number of reflections	30575 *
Completeness [%]	98.4	96.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	318	ammonium dihydrogen phosphate, TRIS, cadmium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 318K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium dihydrogen phosphate	2.0 (M)
2	1	reservoir	Tris-HCl	50 (mM)	pH7.5
3	1	reservoir		2 (mM)