1L0Q

Tandem YVTN beta-propeller and PKD domains from an archaeal surface layer protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	200
Detector technology	CCD
Collection date	1999-11-24
Detector	SBC-2
Wavelength(s)	0.979067,0.979221,0.939270
Spacegroup name	C 1 2 1
Unit cell lengths	188.181, 65.885, 140.030
Unit cell angles	90.00, 124.25, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.217
Rwork	0.214
R-free	0.26300
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	28.428 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.109	0.434
Total number of observations	318719 *
Number of reflections	52881
<I/σ(I)>	14.6	3.1
Completeness [%]	93.7	89
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	298	PEG-8000, potassium chloride, sodium acetate, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	reservoir	PEG8000	20 (%)
3	1	reservoir		0.3 (M)
4	1	reservoir		0.1 (M)	pH3.8