1L0P
CRYSTAL STRUCTURE ANALYSIS OF THE COMPLEX BETWEEN PSYCHROPHILIC ALPHA AMYLASE FROM PSEUDOALTEROMONAS HALOPLANCTIS AND NITRATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 288 |
Detector technology | IMAGE PLATE |
Collection date | 1998-12-28 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.300, 138.400, 114.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.560 - 2.100 |
R-factor | 0.156 |
Rwork | 0.146 |
R-free | 0.18010 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1aqh |
RMSD bond length | 0.006 |
RMSD bond angle | 1.270 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.600 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.150 | |
Total number of observations | 149687 * | |
Number of reflections | 29641 | |
Completeness [%] | 89.3 | 90.5 |
Redundancy | 5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | Aghajari, N., (1996) Protein Sci., 5, 2128. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | MPD | 60-70 (%) | |
2 | 1 | reservoir | HEPES | 0.1 (M) |