1L0B
Crystal Structure of rat Brca1 tandem-BRCT region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2001-05-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.928 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.512, 58.722, 65.327 |
Unit cell angles | 90.00, 111.28, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
R-factor | 0.203 * |
R-free | 0.25800 * |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.610 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.082 * | 0.457 * |
Total number of observations | 55198 * | |
Number of reflections | 17223 | |
Completeness [%] | 97.0 * | 97.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | PEG 8000, Tris-hydrochloride, sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | PEG 8000, Tris-hydrochloride, sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | PEG 8000, Tris-hydrochloride, sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.5 |
2 | 1 | reservoir | 150 (mM) | ||
3 | 1 | reservoir | PEG8000 | 18 (%(w/v)) | |
4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
5 | 1 | drop | protein | 75 (mg/ml) | |
6 | 1 | drop | sodium HEPES | 10 (mM) | pH7.5 |
7 | 1 | drop | 150 (mM) | ||
8 | 1 | drop | dithiothreitol | 5 (mM) |