1KZQ
crystal structure of a parasite protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9197 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 101.840, 101.840, 125.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.062 | 0.424 |
| Number of reflections | 138104 * | |
| <I/σ(I)> | 7.5 | 1.9 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 10.6 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | Ammonium Sulfide, sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | Ammonium Sulfide, sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.8 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |
