1KZP
PROTEIN FARNESYLTRANSFERASE COMPLEXED WITH A FARNESYLATED K-RAS4B PEPTIDE PRODUCT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-07-26 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 |
Unit cell lengths | 171.239, 171.239, 69.361 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.164 |
Rwork | 0.164 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d8d |
RMSD bond length | 0.008 |
RMSD bond angle | 21.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.086 * | 0.569 * |
Total number of observations | 402441 * | |
Number of reflections | 67840 | |
<I/σ(I)> | 21 | 2.47 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.93 | 5.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 17 * | Park, H.W., (1997) Science, 275, 1800. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | FTase | 16 (mg/ml) | |
2 | 1 | drop | 20 (mM) | ||
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | drop | Tris-HCl | 20 (mM) | |
6 | 1 | drop | PEG8000 | 13-15 (%(w/v)) | |
7 | 1 | drop | ammonium acetate | 200 (mM) | |
8 | 1 | reservoir | PEG8000 | 13-15 (%(w/v)) | |
9 | 1 | reservoir | ammonium acetate | 200 (mM) |