1KZH
Structure of a pyrophosphate-dependent phosphofructokinase from the Lyme disease spirochete Borrelia burgdorferi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-10 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.098, 97.926, 148.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.550 |
| Rwork | 0.203 |
| R-free | 0.24300 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.980 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE (SIGMAA) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.670 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.105 | 0.334 |
| Number of reflections | 39787 | |
| <I/σ(I)> | 14.4 | 3.6 |
| Completeness [%] | 96.8 | 98.3 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 * | 278 | 2M Ammonium Sulphate, 0.1M Tris HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 1 (mM) | ||
| 2 | 1 | 1 | pyrophosphate | 2 (mM) | pH7.0 |
| 3 | 1 | 1 | protein | 5.0 (mg/ml) | |
| 4 | 1 | 2 | ammonium sulfate | 2 (M) | |
| 5 | 1 | 2 | Tris | 100 (mM) | pH8.0 |
