1KZG

DbsCdc42(Y889F)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	67.320, 88.190, 232.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.217 *
Rwork	0.217
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Dbs/Cdc42 (1KZ7)
RMSD bond length	0.007
RMSD bond angle	1.290 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.091 *	0.580 *
Total number of observations	225476 *
Number of reflections	43434 *
Completeness [%]	99.6 *	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	18 *	PEG 8000,Tris HCL, NaFormate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	drop	Tris	10 (mM)	pH8.0
3	1	drop		25 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	drop	EDTA	1 (mM)
6	1	reservoir	Tris	50 (mM)	pH7.0
7	1	reservoir	PEG8000	12 (%)
8	1	reservoir	sodium formate	650 (mM)
9	1	reservoir	dithiothreitol	2 (mM)