1KZC
Complex of MBP-C and high-affinity linear trimannose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-02-27 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.800, 75.210, 57.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.850 |
R-factor | 0.213 |
Rwork | 0.210 |
R-free | 0.24200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1rdo |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.068 * | 0.252 * |
Number of reflections | 22973 | |
<I/σ(I)> | 18.8 | 4.9 |
Completeness [%] | 99.9 * | 99.9 * |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20-22 * | PEG 8000, Tris-Cl, NaCl, CaCl2, NaN3, pH 7.4, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12-20 (mg/ml) | |
2 | 1 | drop | Tris-Cl | 10 (mM) | pH7.5 |
3 | 1 | drop | 12 (mM) | ||
4 | 1 | reservoir | PEG8000 | 11-14 (%) | |
5 | 1 | reservoir | Tris-Cl | 100 (mM) | pH7.4 |
6 | 1 | reservoir | 100 (mM) | ||
7 | 1 | reservoir | 12 (mM) | ||
8 | 1 | reservoir | 2 (mM) |