1KZ4
Mutant enzyme W63Y Lumazine Synthase from S.pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 111.941, 145.924, 129.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.400 * - 3.100 |
| R-factor | 0.199 |
| Rwork | 0.195 |
| R-free | 0.23200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.350 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.429 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.110 * | 0.340 * |
| Number of reflections | 19153 | |
| Completeness [%] | 98.6 * | 99.9 * |
| Redundancy | 2.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 295 | citrate, ammonium dihydrogen phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 20 (mM) | pH7.0 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | citrate | 0.1 (M) | pH4.9-5.2 |
| 5 | 1 | reservoir | sodium formate | 1.5 (M) |
