1KYX
Lumazine Synthase from S.pombe bound to carboxyethyllumazine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 111.087, 145.178, 128.554 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.460 * - 2.600 |
R-factor | 0.178 |
Rwork | 0.174 |
R-free | 0.20600 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.440 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.467 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.070 * | 0.420 * |
Number of reflections | 32116 | |
Completeness [%] | 99.5 * | 99.5 * |
Redundancy | 3.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 295 | citrate, ammonium dihydrogen phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 10 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 20 (mM) | pH7.0 |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | reservoir | citrate | 0.1 (M) | pH4.9-5.2 |
5 | 1 | reservoir | sodium formate | 1.5 (M) |