1KYO

YEAST CYTOCHROME BC1 COMPLEX WITH BOUND SUBSTRATE CYTOCHROME C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	277
Detector technology	CCD
Collection date	2001-02-26
Detector	MARRESEARCH
Wavelength(s)	0.931
Spacegroup name	P 1 21 1
Unit cell lengths	147.224, 165.531, 195.889
Unit cell angles	90.00, 104.19, 90.00

Refinement procedure

Resolution	29.640 - 2.970
R-factor	0.228 *
Rwork	0.229
R-free	0.26700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ezv
RMSD bond length	0.008 *
RMSD bond angle	1.420 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	3.080
High resolution limit [Å]	2.970 *	2.970
Rmerge	0.106 *
Number of reflections	175444
<I/σ(I)>	6.8	2.3
Completeness [%]	92.8	76.5
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG4000, 20 mM Tris, 0.05 % Undecyl-maltopyranoside, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K