1KYH
Structure of Bacillus subtilis YxkO, a Member of the UPF0031 Family and a Putative Kinase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-25 |
Detector | SBC-2 |
Wavelength(s) | 0.9795,0.9798,0.94656 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 91.900, 91.900, 170.506 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 1.600 |
R-factor | 0.229 |
Rwork | 0.228 |
R-free | 0.23200 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 1.650 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.146 | 0.546 |
Number of reflections | 48555 * | 4216 * |
<I/σ(I)> | 31 | 2.09 |
Completeness [%] | 97.3 | 86 |
Redundancy | 17 | 4.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.15 M MgCl2, 28% PEG400, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 28 (%) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
4 | 1 | reservoir | 0.15 |