1KYF

AP-2 CLATHRIN ADAPTOR ALPHA-APPENDAGE IN COMPLEX WITH EPS15 DPF PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	110
Detector technology	CCD
Collection date	2000-06-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.10
Spacegroup name	P 1 21 1
Unit cell lengths	40.620, 72.820, 41.860
Unit cell angles	90.00, 99.95, 90.00

Refinement procedure

Resolution	20.000 - 1.220
R-factor	0.153 *
Rwork	0.153
R-free	0.21100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qts
RMSD bond length	0.012
RMSD bond angle	0.031
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.260
High resolution limit [Å]	1.220	1.220
Rmerge	0.059 *	0.388 *
Total number of observations	402957 *
Number of reflections	67213
<I/σ(I)>	15.6	2.3
Completeness [%]	94.5	84
Redundancy	6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein/peptide solution	6 (mg/ml)
2	1	reservoir	ammonium sulfate	1.36-1.49 (M)
3	1	reservoir	HEPES	85-93 (mM)	pH6.8
4	1	reservoir	dioxane	8.5-9.3 (%)
5	1	reservoir	glycerol	0-5 (%)