1KYD
AP-2 CLATHRIN ADAPTOR ALPHA-APPENDAGE IN COMPLEX WITH EPSIN DPW PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-11-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.780, 71.880, 41.670 |
| Unit cell angles | 90.00, 99.97, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.000 |
| R-factor | 0.178 * |
| Rwork | 0.178 |
| R-free | 0.22900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qts |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 * | 0.285 * |
| Total number of observations | 59931 * | |
| Number of reflections | 16055 | |
| <I/σ(I)> | 12.4 | 3.7 |
| Completeness [%] | 93.3 | 84.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein/peptide solution | 6 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.36-1.49 (M) | |
| 3 | 1 | reservoir | HEPES | 85-93 (mM) | pH6.8 |
| 4 | 1 | reservoir | dioxane | 8.5-9.3 (%) | |
| 5 | 1 | reservoir | glycerol | 0-5 (%) |
