1KYA

ACTIVE LACCASE FROM TRAMETES VERSICOLOR COMPLEXED WITH 2,5-XYLIDINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-08-22
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	87.720, 110.520, 123.201
Unit cell angles	90.00, 103.44, 90.00

Refinement procedure

Resolution	35.000 - 2.400
R-factor	0.253 *
Rwork	0.253
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a65
RMSD bond length	0.007
RMSD bond angle	1.470
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	83644
<I/σ(I)>	9.7
Completeness [%]	93.6	71.6
Redundancy	22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Bertrand, T., (2002) Acta Crystallogr., Sect.D, 58, 319. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	reservoir	PEG8000	18 (%(w/v))
3	1	reservoir	zinc acetate	0.2 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)	pH6.5