1KXW

ANALYSIS OF THE STABILIZATION OF HEN LYSOZYME WITH THE HELIX DIPOLE AND CHARGED SIDE CHAINS

Experimental procedure

Temperature [K]	295
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	79.160, 79.160, 37.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.750 *
R-factor	0.168
Rwork	0.168
Starting model (for MR)	1rfp
RMSD bond length	0.009 *
RMSD bond angle	1.448 *
Data reduction software	PROCESS ((SCALE))
Data scaling software	PROCESS ((SCALE))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.020
High resolution limit [Å]	1.750 *	1.960
Rmerge	0.037 *	0.183
Number of reflections	11948 *
Completeness [%]	93.2 *	88.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *		50 MM ACETATE AT PH 4.7 CONTAINING 0.9 M NACL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	50 (mM)	pH4.7
2	1	reservoir		0.9-1.2 (M)
3	1	drop	protein	9.3*10-7 (M)
4	1	drop	sodium acetate	0.1 (M)	with HCl, pH5.5