1KXV
Camelid VHH Domains in Complex with Porcine Pancreatic alpha-Amylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 57.568, 60.990, 107.333 |
Unit cell angles | 98.78, 100.88, 101.14 |
Refinement procedure
Resolution | 30.000 * - 1.700* |
R-factor | 0.205 * |
Rwork | 0.205 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fjh PDB ENTRY 1QD0 |
RMSD bond length | 0.020 |
RMSD bond angle | 25.800 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.690 |
High resolution limit [Å] | 1.700 * | 1.700 * |
Rmerge | 0.074 | 0.137 |
Total number of observations | 716803 * | |
Number of reflections | 175069 | |
<I/σ(I)> | 4.6 | 2 |
Completeness [%] | 99.8 * | 99.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | PEG4000 32%, Na citrate 0.1M & Ammonium Acetate 0.2M, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 50 (mM) | pH7.5 |
2 | 1 | drop | 100 (mM) | ||
3 | 1 | reservoir | PEG4000 | 32 (%) | |
4 | 1 | reservoir | sodium citrate | 0.1 (M) | |
5 | 1 | reservoir | ammonium acetate | 0.2 (M) | pH5.0 |