1KXT

Camelid VHH Domains in Complex with Porcine Pancreatic alpha-Amylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	52.780, 286.850, 65.980
Unit cell angles	90.00, 93.73, 90.00

Refinement procedure

Resolution	20.000 * - 2.000
R-factor	0.203 *
Rwork	0.203
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fjh PDB ENTRY 1QDO
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.070	0.137 *
Total number of observations	456505 *
Number of reflections	78282 *
<I/σ(I)>	7
Completeness [%]	51.0 *	50

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	293	PEG 20000 15%, Malate imidazole 0.2M, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	50 (mM)	pH7.5
2	1	drop		100 (mM)
3	1	reservoir	PEG20000	10-15 (%)
4	1	reservoir	imidazole malate	0.1-0.2 (M)	pH8.0