1KXP
CRYSTAL STRUCTURE OF HUMAN VITAMIN D-BINDING PROTEIN IN COMPLEX WITH SKELETAL ACTIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.758, 87.364, 162.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.100 |
| Rwork | 0.217 |
| R-free | 0.23600 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 20.640 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.093 | 0.286 |
| Total number of observations | 1064049 * | |
| Number of reflections | 67458 | |
| <I/σ(I)> | 23.4 | 11.2 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 15.8 | 11.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 4 * | Peg 8K 12%, Magnesium Acetate 200mM, Sodium Cacodylate 100 mM, Glycerol 20%, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 100 (mM) | pH7.6 |
| 2 | 1 | drop | ATP | 0.6 (mM) | |
| 3 | 1 | drop | beta-mercaptoethanol | 0.5 (mM) | |
| 4 | 1 | drop | 0.2 (mM) | ||
| 5 | 1 | drop | protein | 20 (mg/ml) | |
| 6 | 1 | reservoir | PEG12000 | 11 (%) | |
| 7 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 8 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.6 |
| 9 | 1 | reservoir | glycerol | 20 (%) |
