1KXH
Crystal structure of the complex between an inactive mutant of psychrophilic alpha-amylase (D174N) and acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.100, 139.900, 115.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.700 - 2.300 |
| Rwork | 0.138 |
| R-free | 0.18800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1aqm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.092 | 0.309 |
| Number of reflections | 24442 | |
| <I/σ(I)> | 11.2 | 4.7 |
| Completeness [%] | 95.6 | 91.1 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MPD, hepes, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
