1KXF

SINDBIS VIRUS CAPSID, (WILD-TYPE) RESIDUES 1-264, TETRAGONAL CRYSTAL FORM (FORM II)

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1992-02-26
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	57.000, 57.000, 109.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.400 *
R-factor	0.19
Rwork	0.190
R-free	0.28400
RMSD bond length	0.014
RMSD bond angle	27.500 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.042	0.134 *
Total number of observations	19176 *
Number of reflections	7908
Completeness [%]	96.2	90.4 *
Redundancy	2.42

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.5-7.5 (mg/ml)
2	1	reservoir	PEG8000	6-8 (%(w/v))
3	1	reservoir	Tris-HCl	50-300 (mM)