1KX9
ANTENNAL CHEMOSENSORY PROTEIN A6 FROM THE MOTH MAMESTRA BRASSICAE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.600, 49.700, 50.300 |
| Unit cell angles | 90.00, 110.10, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 1.650 |
| Rwork | 0.204 |
| R-free | 0.20400 * |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 19.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 1.750 |
| High resolution limit [Å] | 1.600 * | 1.600 * |
| Rmerge | 0.038 | 0.245 |
| Number of reflections | 25956 | |
| <I/σ(I)> | 11.8 | 7.2 |
| Completeness [%] | 93.3 * | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 293 | Campanacci, V., (2001) Acta Crystallogr., Sect.D, 57, 137. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 46 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH8.0 |
| 3 | 1 | reservoir | PEG5000 MME | 36 (%) | |
| 4 | 1 | reservoir | sodium acetate | 0.2 (M) | pH5.5 |
