1KX8
Antennal Chemosensory Protein A6 from Mamestra brassicae, tetragonal form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97888, 0.97909, 0.91839 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.406, 69.406, 79.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.800 |
| Rwork | 0.265 |
| R-free | 0.26500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 18.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | 0.320 |
| Number of reflections | 5096 | |
| <I/σ(I)> | 22 | 4.5 |
| Completeness [%] | 99.0 * | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 293 | Campanacci, V., (2001) Acta Crystallogr., Sect.D, 57, 137. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 46 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH8.0 |
| 3 | 1 | reservoir | PEG5000 MME | 36 (%) | |
| 4 | 1 | reservoir | sodium acetate | 0.2 (M) | pH5.5 |
